Classification of Grass Fructans by 13C NMR Spectroscopy.
نویسندگان
چکیده
منابع مشابه
Supramolecular interactions probed by 13C-13C solid-state NMR spectroscopy.
We present a robust solid-state NMR approach for the accurate determination of molecular interfaces in insoluble and noncrystalline protein-protein complexes. The method relies on the measurement of intermolecular (13)C-(13)C distances in mixtures of [1-(13)C]glucose- and [2-(13)C]glucose-labeled proteins. We have applied this method to Parkinson's disease-associated α-synuclein fibrils and fou...
متن کاملProbing molecular motion by double-quantum (13C,13C) solid-state NMR spectroscopy: application to ubiquitin.
We demonstrate the use of two-dimensional ((13)C,(13)C) double-quantum spectroscopy to detect molecular dynamics by solid-state NMR. Data collected on tyrosine-ethylester (TEE) are in line with previously determined ((1)H,(13)C) order parameters. Application of these experiments to microcrystalline ubiquitin reveals the presence of dynamics on millisecond or faster time scales and differences i...
متن کامل13C NMR Spectroscopy of Labeled Pyridoxal Y-Phosphate
Pyridoxal 5’.phosphate labeled to the extent of 90%’ with 13C in the 4’ (aldehyde) and 5’ (methylene) positions has been synthesized. 13C NMR spectra of this material and of natural abundance pyridoxal 5’.phosphate are reported, as well as r3C NMR spectra of the Schiff base formed by reaction of pyridoxal 5’.phosphate with n-butylamine, the secondary amine formed by reduction of this Schiff bas...
متن کاملLocalized in vivo 13C NMR spectroscopy of the brain.
Localized (13)C NMR spectroscopy provides a new investigative tool for studying cerebral metabolism. The application of (13)C NMR spectroscopy to living intact humans and animals presents the investigator with a number of unique challenges. This review provides in the first part a tutorial insight into the ingredients required for achieving a successful implementation of localized (13)C NMR spe...
متن کامل2H-DNP-enhanced 2H-13C solid-state NMR correlation spectroscopy.
Perdeuteration of biological macromolecules for magic angle spinning solid-state NMR spectroscopy can yield high-resolution (2)H-(13)C correlation spectra and the method is therefore of great interest for the structural biology community. Here we demonstrate that the combination of sample deuteration and dynamic nuclear polarization yields resolved (2)H-(13)C correlation spectra with a signal e...
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1990
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.44-0158